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Medicago truncatula Mutant Database |
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The Samuel Roberts Noble Foundation, funded in part by a research grant from the National Science Foundation, has generated a significant number of Medicago truncatula Tnt1 mutant lines. These lines are now available to the scientific community for research purposes.
To assist with line maintenance, seed propagation, shipping and handling, the cost of each ordered line will be $100 USD.
Seeds of the mutant lines can be ordered online. Registration is required to complete the ordering procedure. You MUST sign in before place mutant orders.
Once your request form is received, we will send you a Materials Transfer Agreement (MTA) to be signed by your institution as well as an invoice for payment. As soon as we receive payment and an executed MTA, we will send you 10-12 R0 or 15-20 R1 seeds for each requested line.
R0 seeds are seeds of regenerated plants after self pollination (heterozygous for all inserts). R1 seeds are bulked seeds from multiple R1 plants (generated after self pollination of plants germinated from R0 seeds). Both R0 and R1 seeds will generate segregating progeny.
Please note that seeds are available for most but not all Tnt1 lines. Some lines did not produce seeds or seeds are not available in sufficient quantity to enable distribution at this time. In the latter case, seed will be amplified before filling orders, which will take several months.
Every reasonable effort will be made to provide you with seeds from the lines you request; however, inadvertent errors in cataloging or handling of seeds may occur. It is advisable that you confirm the Tnt1 insertion in your gene of interest before starting your research. The terms and conditions concerning the transfer and your use of the Medicago truncatula Tnt1 mutant lines, including limitations of liability, will be governed by the MTA.